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11 October 1993 Electroporation visualized under a multishot pulsed laser fluorescence microscope system
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Abstract
We describe a new fluorescence microscope system, which is the third generation of our pulsed-laser microscope systems developed for the purpose of capturing rapid cellular phenomena. Time resolution of this latest version is supported by the combination of a Q- switched Nd:YAG laser producing a burst of 4 pulses and a large format framing camera. We obtain series images at intervals on the order of 10 microsecond(s) with exposure times of 30 ns. With this multi-shot pulsed laser fluorescence microscope system, we examined the behavior of the transmembrane potential in a sea urchin egg under an intense electric field. Irreversible process of cell electroporation was revealed in serial images taken under a single electric pulse of microsecond duration.
© (1993) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Hiroyasu Itoh, Irene I. K. Yu, Masahiro Hibino, Tsuyoshi Hayakawa, and Kazuhiko Kinosita Jr. "Electroporation visualized under a multishot pulsed laser fluorescence microscope system", Proc. SPIE 2002, Ultrahigh- and High-Speed Photography, Videography, and Photonics '93, (11 October 1993); https://doi.org/10.1117/12.161357
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