Fluorescence lifetime-based sensing of zinc in solution

Richard B. Thompson; Marcia W. Patchan

doi:10.1117/12.170676

10 March 1994 Fluorescence lifetime-based sensing of zinc in solution

Richard B. Thompson, Marcia W. Patchan

Author Affiliations +

Proceedings Volume 2068, Chemical, Biochemical, and Environmental Fiber Sensors V; (1994) https://doi.org/10.1117/12.170676
Event: Optical Tools for Manufacturing and Advanced Automation, 1993, Boston, MA, United States

Abstract

Recently, we have described a fiber optic biosensor specific for zinc that transduces the presence of the metal as a shift in the emission of a fluorescent sulfonamide inhibitor that binds to a metalloenzyme, human erythrocyte carbonic anhydrase, if and only if the metal is present. Due to fiber photoluminescence and calibration issues, we decided to determine if comparable results could be obtained by sensing based on fluorescence lifetime changes, as recently described by Demas, Wolfbeis, Lakowicz, and others. Results of these experiments are included, as well as a discussion of the dynamic range of the method.

Citation Download Citation

Richard B. Thompson, Richard B. Thompson, Marcia W. Patchan, Marcia W. Patchan, "Fluorescence lifetime-based sensing of zinc in solution", Proc. SPIE 2068, Chemical, Biochemical, and Environmental Fiber Sensors V, (10 March 1994); doi: 10.1117/12.170676; https://doi.org/10.1117/12.170676

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