1 March 1994 Distribution of photosensitizers between tumor cells and tumor infiltrating host cells
Author Affiliations +
Proceedings Volume 2078, Photodynamic Therapy of Cancer; (1994) https://doi.org/10.1117/12.168679
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary
Photofrin levels in different cellular populations constituting a murine FsaR fibrosarcoma were measured by flow cytometry. Both myeloid and lymphoid populations associated with the tumor were found to accumulate more photosensitizer on a per cell basis, on average, than the malignant cells. Macrophages, identified by the F4/80 antigen, exceeded other myeloid cells in Photofrin accumulation. It is shown that one of the factors involved is the variability in the photosensitizer content in cells located at different distances from the nearest blood vessel. This was investigated by a flow cytometry technique with the fluorescent stain Hoechst 33342, used to distinguish cells depending on their proximity to the tumor vasculature.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Mladen Korbelik, Mladen Korbelik, Gorazd Krosl, Gorazd Krosl, } "Distribution of photosensitizers between tumor cells and tumor infiltrating host cells", Proc. SPIE 2078, Photodynamic Therapy of Cancer, (1 March 1994); doi: 10.1117/12.168679; https://doi.org/10.1117/12.168679

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