15 January 1994 Correction of blood perfusion influences on NADH fluorescence spectroscopic measurements
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Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166826
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary
In vivo measurement of NADH autofluorescence is strongly disturbed by changes in the Hb absorption of excitation and fluorescence light. An approach to this problem is based on the diffuse reflectance measurement of 805 nm (isobestic point of Hb/HbO2). According a Kubelka-Munk calculation an increase in Hb/HbO2 concentration leads to a decrease of diffuse reflectance. This gives correction terms for the measured autofluorescence data. An equipment to perform such measurements will be presented.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Wolfgang Hoehne, Wolfgang Hoehne, Werner Schramm, Werner Schramm, } "Correction of blood perfusion influences on NADH fluorescence spectroscopic measurements", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); doi: 10.1117/12.166826; https://doi.org/10.1117/12.166826

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