Sergei R. Utz Institute of Rural Hygiene and Occupational Diseases (Russia) Joachim Barth,Peter Knuschke Medical Academy Carl Gustav Carus (Germany) Yurii P. Sinichkin Institute of Rural Hygiene and Occupational Diseases (Russia)
The advantages of optical spectroscopy in skin are discussed. The basis for fluorescence spectroscopy is briefly presented. The potential for in vitro and in vivo fluorescence spectroscopy of biotissues is significant, but not yet well developed. With the help of fluorescence spectroscopy and layer-by-layer skin surface strippings technique the important knowledge for autofluorescence spectra from different skin layers have been obtained. Resolution of autofluorescence spectra into discrete excitation-emission bands is presented, related to various skin fluorophores.