Tissue diagnosis using autofluorescence

Wolfgang Lohmann; Wolf-Bernhard Schill; Dieter Bucher; Theofried Peters; M. Nilles; Andreas Schulz; Rainer M. Bohle; Werner Schramm

doi:10.1117/12.166825

15 January 1994 Tissue diagnosis using autofluorescence

Wolfgang Lohmann, Wolf-Bernhard Schill, Dieter Bucher, Theofried Peters, M. Nilles, Andreas Schulz, Rainer M. Bohle, Werner Schramm

Author Affiliations +

Proceedings Volume 2081, Optical Biopsy; (1994) https://doi.org/10.1117/12.166825
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The fluorescence response of biological tissue (excited at 366 nm or 337 nm) depends on its condition: the intensity is rather low in cancer tissue and slightly higher at the edge of the tumor than in healthy tissue. This technique is non-invasive, non-destructive and is based on the native fluorophore NADH. Fluorescence images obtained with unstained, unfixed cryosections match the histological images. Since the cryosections can be prepared and evaluated in less than 5 min, this technique can also be used as a fast cut technique to determine e.g. the diagnosis of a biopsy sample. Additional investigations have shown that the fluorophore seems to be attached to elastic fibers.

Citation Download Citation

Wolfgang Lohmann, Wolf-Bernhard Schill, Dieter Bucher, Theofried Peters, M. Nilles, Andreas Schulz, Rainer M. Bohle, and Werner Schramm "Tissue diagnosis using autofluorescence", Proc. SPIE 2081, Optical Biopsy, (15 January 1994); https://doi.org/10.1117/12.166825

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