Dependency of subcellular reactions during PDT on the metabolic state of cell cultures probed by different microscopic techniques

Angelika C. Rueck; Herbert Schneckenburger; Wolfgang S. L. Strauss; Michael H. Gschwend; Gerd C. Beck; Karin Kunzi-Rapp; Rudolf W. Steiner

doi:10.1117/12.167424

1 February 1994 Dependency of subcellular reactions during PDT on the metabolic state of cell cultures probed by different microscopic techniques

Angelika C. Rueck, Herbert Schneckenburger, Wolfgang S. L. Strauss, Michael H. Gschwend, Gerd C. Beck, Karin Kunzi-Rapp, Rudolf W. Steiner

Author Affiliations +

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167424
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Various microscopic techniques were used to study the dependency of photodynamically induced subcellular reactions on the metabolic state of cell cultures. TPPS₄ and AlS_2-3Pc were incubated in RR 1022 epithelial cells with varying cell density. To attain almost isolated cells (low cell density) or confluent growing cells (high cell density) 25 cells/mm² or 500 cells/mm² were seeded, respectively. Low cell density irradiation with blue light led to a change in the initial cytoplasmatic fluorescence pattern. For both sensitizers, TPPS₄ as well as AlS_2-3, a fluorescence relocalization and fluorescence intensity increase could be detected, moreover in the case of TPPS₄ a fluorescence formation in the nucleus and nucleoli were detected. In contrast, for confluent growing cells no redistribution was observed.

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Angelika C. Rueck, Herbert Schneckenburger, Wolfgang S. L. Strauss, Michael H. Gschwend, Gerd C. Beck, Karin Kunzi-Rapp, and Rudolf W. Steiner "Dependency of subcellular reactions during PDT on the metabolic state of cell cultures probed by different microscopic techniques", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167424

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