High-resolution microscopic techniques for probing subcellular changes during photodynamic therapy

Michael H. Gschwend; Herbert Schneckenburger; Wolfgang S. L. Strauss; Angelika C. Rueck; Rudolf W. Steiner

doi:10.1117/12.167423

1 February 1994 High-resolution microscopic techniques for probing subcellular changes during PDT

Michael H. Gschwend, Herbert Schneckenburger, Wolfgang S. L. Strauss, Angelika C. Rueck, Rudolf W. Steiner

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167423
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Subcellular changes during photodynamic therapy (PDT) were probed simultaneously with two different microscopic techniques. These techniques include differential interference contrast (DIC) microscopy and fluorescence microscopy, in combination with video-contrast enhancement, averaging techniques, and digital image processing. The different cell organelles were identified by specific markers, such as rhodamine 123 and acridine orange. Subcellular morphological changes in fibroblastoid cells (3T3) and epithelial cells (RR 1022), incubated with the photosensitizer meso-tetra(4-sulfonatophenyl)porphyrin (TPPS₄), were investigated during PDT treatment and correlated with the fluorescence pattern.

Citation Download Citation

Michael H. Gschwend, Herbert Schneckenburger, Wolfgang S. L. Strauss, Angelika C. Rueck, and Rudolf W. Steiner "High-resolution microscopic techniques for probing subcellular changes during PDT", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167423

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