High-resolution microscopic techniques for probing subcellular changes during photodynamic therapy

Michael H. Gschwend; Herbert Schneckenburger; Wolfgang S. L. Strauss; Angelika C. Rueck; Rudolf W. Steiner

doi:10.1117/12.167423

1 February 1994 High-resolution microscopic techniques for probing subcellular changes during PDT

Michael H. Gschwend; Herbert Schneckenburger; Wolfgang S. L. Strauss; Angelika C. Rueck; Rudolf W. Steiner

Author Affiliations +

Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167423
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Subcellular changes during photodynamic therapy (PDT) were probed simultaneously with two different microscopic techniques. These techniques include differential interference contrast (DIC) microscopy and fluorescence microscopy, in combination with video-contrast enhancement, averaging techniques, and digital image processing. The different cell organelles were identified by specific markers, such as rhodamine 123 and acridine orange. Subcellular morphological changes in fibroblastoid cells (3T3) and epithelial cells (RR 1022), incubated with the photosensitizer meso-tetra(4-sulfonatophenyl)porphyrin (TPPS₄), were investigated during PDT treatment and correlated with the fluorescence pattern.

Citation Download Citation

Michael H. Gschwend, Michael H. Gschwend, Herbert Schneckenburger, Herbert Schneckenburger, Wolfgang S. L. Strauss, Wolfgang S. L. Strauss, Angelika C. Rueck, Angelika C. Rueck, Rudolf W. Steiner, Rudolf W. Steiner, } "High-resolution microscopic techniques for probing subcellular changes during PDT", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); doi: 10.1117/12.167423; https://doi.org/10.1117/12.167423

ACCESS THE FULL ARTICLE

PERSONAL SIGN IN
Full access may be available with your subscription

Email or Username Forgot your username?

Password Forgot your password?

Show

Remember Email/Username on this computer

Remember Password

No SPIE account? Create an account
or Institutional Sign In via Shibboleth

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $14.40

Non-members: $18.00 ADD TO CART

Show All Keywords

Keywords/Phrases

Search In:

Publication Years