Methods and application of time-resolved fluorescence microscopy

Harold K. Seidlitz; Jurina M. Wessels

doi:10.1117/12.167426

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1 February 1994 Methods and application of time-resolved fluorescence microscopy

Harold K. Seidlitz; Jurina M. Wessels

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Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167426
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

The combination of fluorescence microscopy and time-resolved techniques opens up new possibilities in studying pharmacokinetics and localization of various drugs on a cellular scale. The fluorescence decay time of many molecules is modulated by the physical and chemical properties of the microenvironment, such as viscosity, polarity, pH-value, etc. The alternation of the decay time can also be used to pursue chemical transformations of a drug like degradation, monomerization, or protonation. This paper presents applications of this method from the field of photosensitizing dyes used in photodynamic therapy.

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Harold K. Seidlitz and Jurina M. Wessels "Methods and application of time-resolved fluorescence microscopy", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167426

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