Video microscopy of vascular damage during PDT

Sol Kimel; Varda Gottfried

doi:10.1117/12.167425

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1 February 1994 Video microscopy of vascular damage during photodynamic therapy

Sol Kimel; Varda Gottfried

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Proceedings Volume 2083, Microscopy, Holography, and Interferometry in Biomedicine; (1994) https://doi.org/10.1117/12.167425
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary

Abstract

Photodynamic therapy (PDT) was performed in the chorioallantoic membrane (CAM) of the chick embryo. This is a convenient model to study vascular effects. PDT-induced damage was monitored continuously during irradiation and recorded on a VCR driven by a PC. Time-lapse video documentation provided a detailed view of the entire process: tumor growth, angiogenesis, vascularization, PDT, and tumor regression. Our particular interest was in resolving the controversy regarding the primary mechanism in PDT, as to whether the predominant damage is to the parenchymal tumor cells or to the vascular endothelium. Image analysis techniques enabled us to follow quantitatively changes occurring during the process. These changes included alternations in blood vessels (color and morphology) and in tumors (tumor area).

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Sol Kimel and Varda Gottfried "Video microscopy of vascular damage during photodynamic therapy", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994); https://doi.org/10.1117/12.167425

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