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1 February 1994 Video microscopy of vascular damage during photodynamic therapy
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Photodynamic therapy (PDT) was performed in the chorioallantoic membrane (CAM) of the chick embryo. This is a convenient model to study vascular effects. PDT-induced damage was monitored continuously during irradiation and recorded on a VCR driven by a PC. Time-lapse video documentation provided a detailed view of the entire process: tumor growth, angiogenesis, vascularization, PDT, and tumor regression. Our particular interest was in resolving the controversy regarding the primary mechanism in PDT, as to whether the predominant damage is to the parenchymal tumor cells or to the vascular endothelium. Image analysis techniques enabled us to follow quantitatively changes occurring during the process. These changes included alternations in blood vessels (color and morphology) and in tumors (tumor area).
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sol Kimel and Varda Gottfried "Video microscopy of vascular damage during photodynamic therapy", Proc. SPIE 2083, Microscopy, Holography, and Interferometry in Biomedicine, (1 February 1994);

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