1 February 1994 Liquid chromatography combined with visible diode-laser-induced fluorescence detection for photosensitizers
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Proceedings Volume 2084, Biomedical Optoelectronic Devices and Systems; (1994) https://doi.org/10.1117/12.167312
Event: Europto Biomedical Optics '93, 1993, Budapest, Hungary
Abstract
A combination of conventional-size liquid chromatography and visible diode-laser induced fluorescence (DIO-LIF) detection has been developed and applied for the detection of a model photosensitizer, AlPcS2, in biological samples. Conventional fluorimetry was found to give unsatisfactory information. A detection limit of 2.1 X 10-12 M (S/N equals 3) was obtained for AlPcS2 in both rat urine and faeces extracts using the DIO-LIF system, 20-fold lower than obtained with a conventional fluorescence detection system, when used in combination with chromatography.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Arjan J.G. Mank, Arjan J.G. Mank, Cees Gooijer, Cees Gooijer, Henk Lingeman, Henk Lingeman, Nel H. Velthorst, Nel H. Velthorst, Udo A. T. Brinkman, Udo A. T. Brinkman, } "Liquid chromatography combined with visible diode-laser-induced fluorescence detection for photosensitizers", Proc. SPIE 2084, Biomedical Optoelectronic Devices and Systems, (1 February 1994); doi: 10.1117/12.167312; https://doi.org/10.1117/12.167312
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