Protocol for the onfrared assignment of conformation sensitive protein bands

Robert J. Jakobsen; Jay R. Powell

doi:10.1117/12.166683

31 January 1994 Protocol for the infrared assignment of conformation sensitive protein bands

Robert J. Jakobsen, Jay R. Powell

Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166683
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

Infrared spectroscopy is being increasingly used to determine the secondary structure (conformation) of proteins. Therefore it is of critical importance to correctly assign the conformation sensitive bands of proteins. Unfortunately, protein assignments are often based on a `hit or miss' proposition without sufficient experimental verification. The following paper outlines a protocol for assigning the conformation sensitive bands of proteins based on an easy, fast experimental procedure which exploits known conformational effects of different solvents upon the protein secondary structure.

Citation Download Citation

Robert J. Jakobsen and Jay R. Powell "Protocol for the infrared assignment of conformation sensitive protein bands", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166683

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