31 January 1994 Protocol for the infrared assignment of conformation sensitive protein bands
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Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166683
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada
Abstract
Infrared spectroscopy is being increasingly used to determine the secondary structure (conformation) of proteins. Therefore it is of critical importance to correctly assign the conformation sensitive bands of proteins. Unfortunately, protein assignments are often based on a `hit or miss' proposition without sufficient experimental verification. The following paper outlines a protocol for assigning the conformation sensitive bands of proteins based on an easy, fast experimental procedure which exploits known conformational effects of different solvents upon the protein secondary structure.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Robert J. Jakobsen, Jay R. Powell, "Protocol for the infrared assignment of conformation sensitive protein bands", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); doi: 10.1117/12.166683; https://doi.org/10.1117/12.166683
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