31 January 1994 Structural characterization and nanometer-scale domain formation in phospholipid model membranes by infrared spectroscopy and scanning tunneling microscopy
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Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166605
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada
Abstract
Research in this laboratory is presently being conducted towards the structural characterization of well-ordered molecular monolayers using an integrated spectroscopic/imaging approach. We are currently using infrared spectroscopy (IR) in combination with scanning tunneling microscopy (STM) on both Langmuir-Blodgett (L-B) and self-assembled (SA) monolayer films in order to obtain compositional, conformational, and topographic information about these films. We have constructed organized lipid bilayer assemblies using a combination of SA and L-B methods which were studied using this combined IR/STM approach. These studies showed acyl chain tilt angles of 5 - 10 degree(s) within the DPPG monolayer by IR and STM. In addition, nanometer-size DPPG with hexagonal-type grain boundaries, and molecular resolution of phosphoglycerol headgroup structure within these domains, have been observed by STM. Phospholipid headgroup areas are in excellent agreement with previous x-ray diffraction studies of this system.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Brian W. Gregory, Brian W. Gregory, Richard A. Dluhy, Richard A. Dluhy, Lawrence A. Bottomley, Lawrence A. Bottomley, } "Structural characterization and nanometer-scale domain formation in phospholipid model membranes by infrared spectroscopy and scanning tunneling microscopy", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); doi: 10.1117/12.166605; https://doi.org/10.1117/12.166605
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