Use of particle beam LC/FTIR spectrometry for the structural analysis of globular proteins: preliminary studies with beta-lactoglobulin

Vincent E. Turula; James A. de Haseth

doi:10.1117/12.166707

31 January 1994 Use of particle beam LC/FTIR spectrometry for the structural analysis of globular proteins: preliminary studies with beta-lactoglobulin

Vincent E. Turula, James A. de Haseth

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Proceedings Volume 2089, 9th International Conference on Fourier Transform Spectroscopy; (1994) https://doi.org/10.1117/12.166707
Event: Fourier Transform Spectroscopy: Ninth International Conference, 1993, Calgary, Canada

Abstract

The Particle Beam apparatus can be used to desolvate proteins in preparation of IR measurements. Several experiments have been designed to determine whether the operation of the particle beam apparatus causes alternation to the complex structural features of globular proteins, and whether it produces a solid-state spectrum representative of the aqueous solution structure. (beta) -Lactoglobulin ((beta) LG), a milk protein with a structure well- characterized by infrared spectrometry, was chosen for this study. It is shown here that the structural integrity of this protein is maintained when nebulized, desolvated from solution, and deposited onto the IR-substrate. It is also possible to induce structural changes in proteins with the particle beam and a carrier-liquid concentration gradient.

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Vincent E. Turula and James A. de Haseth "Use of particle beam LC/FTIR spectrometry for the structural analysis of globular proteins: preliminary studies with beta-lactoglobulin", Proc. SPIE 2089, 9th International Conference on Fourier Transform Spectroscopy, (31 January 1994); https://doi.org/10.1117/12.166707

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KEYWORDS

Proteins

Particle beams

Spectroscopy

Infrared spectroscopy

Infrared radiation

Liquids

FT-IR spectroscopy

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