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23 June 1994 Matrix metalloproteinase expression in excimer laser wounded rabbit corneas
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Proceedings Volume 2126, Ophthalmic Technologies IV; (1994) https://doi.org/10.1117/12.178583
Event: OE/LASE '94, 1994, Los Angeles, CA, United States
Abstract
This study was performed to obtain information about matrix metalloproteinase (MMP) expression in excimer-wounded corneas and to determine whether MMPs expression correlates with the depth of the ablation. 6-mm excimer keratectomy (60 or 180 micrometers ) was performed using the 193-mm ArF excimer laser on 12 NZW rabbits. Corneas treated with mechanical epithelial debridement and untreated corneas served as controls. Rabbits were killed at 20 and 30 hr after laser ablation. Zymography after SDS extraction was performed on regenerated central epithelium and the central stroma to determine MMPs expression. We observed enzymatic activity of a 92 KDa band in the epithelium of excimer-ablated corneas but not in that following debridement wounds and untreated controls. The expression of the 92 KDa MMP was most pronounced with the deeper excimer ablation. A 72 KDa band of enzymatic activity present in the stroma of all treated and control eyes was also seen in the epithelium of excimer-ablated corneas. These proteolytic enzymes may play an important role in wound healing and remodelling after excimer keratectomy.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Taewon Hahn, Wallace Chamon, Yonja Akova, Walter J. Stark M.D., William G. Stetler-Stevenson, and Dimitri T. Azar M.D. "Matrix metalloproteinase expression in excimer laser wounded rabbit corneas", Proc. SPIE 2126, Ophthalmic Technologies IV, (23 June 1994); https://doi.org/10.1117/12.178583
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