Selective treatment of atherosclerosis using photodynamic therapy in the Yucatan miniswine: preliminary results

York N. Hsiang M.D.; M. Teresa Crespo; G. T. M. Houston M.D.; Eleanor C. To; Mary E. Todd; Mohammed S. Sobeh; Stephen E. Greenwald; Robert D. Bower

doi:10.1117/12.179930

12 July 1994 Selective treatment of atherosclerosis using photodynamic therapy in the Yucatan miniswine: preliminary results

York N. Hsiang M.D., M. Teresa Crespo, G. T. M. Houston M.D., Eleanor C. To, Mary E. Todd, Mohammed S. Sobeh, Stephen E. Greenwald, Robert D. Bower

Author Affiliations +

Proceedings Volume 2130, Diagnostic and Therapeutic Cardiovascular Interventions IV; (1994) https://doi.org/10.1117/12.179930
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Photodynamic therapy (PDT) represents a novel method of selectively treating atherosclerosis using a combination of photosensitizer drug, low power laser light, and molecular oxygen. In this preliminary study, PDT was used to treat atherosclerotic lesions in a miniswine model. Yucatan miniswine weighing between 20-30 kg, were rendered atherosclerotic by a combination of balloon endothelial injury and dietary supplementation with 2% cholesterol and 15% lard diet for 7 weeks. Following this, miniswine were given a porphyrin-type photosensitizer, Photofrin 2.5 mg/kg IV. Twenty-four hours after receiving Photofrin, swine received a general anesthetic and the infrarenal abdominal aorta was exposed. Through a longitudinal aortotomy, the posterior aortic wall was irradiated with 630 nm laser light at one of the following light doses: 60, 120, and 240 J/cm². Four weeks after PDT, swine were killed and perfusion- fixed with glutaraldehyde. Light microscopy showed a decrease in intimal thickness for all light doses. Decreased cellular elements were seen in the irradiated zones as the laser power was increased. Non-irradiated sites showed typical atherosclerotic lesions with foam cells, fibrosis and calcification. This study demonstrated the feasibility of using PDT for atherosclerotic lesions.

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York N. Hsiang M.D., M. Teresa Crespo, G. T. M. Houston M.D., Eleanor C. To, Mary E. Todd, Mohammed S. Sobeh, Stephen E. Greenwald, and Robert D. Bower "Selective treatment of atherosclerosis using photodynamic therapy in the Yucatan miniswine: preliminary results", Proc. SPIE 2130, Diagnostic and Therapeutic Cardiovascular Interventions IV, (12 July 1994); https://doi.org/10.1117/12.179930

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