The paper focuses on the moire technique as a tool of biomedical measurement. The real advantage of moire measurement in muscle pulsing lies in solving no contact requirement problems for which the measured system needs whole field monitoring and quick response. With the moire optical measurement system and a video camera we obtained the detail analysis of the behavior of muscle pulsing. Post-processing procedures were performed by an image processing system. The moire system was compared with a fiber optical sensor, our fiber sensing head is multiple fiber bounding with novel devices to prevent the confusion on front slope and back slope in the relation of displacement and signal intensity. The fiber optical sensor gives the reference displacement and velocity value of the major point of the muscle. In this study we also discuss the possible applications of the moire auto-measurement system in muscle pulsing.