Substrate-induced quenching of a fluorophore and its application in biosensor development

Ashutosh Sharma; Nigel S. M. Quantrill

doi:10.1117/12.180764

28 July 1994 Substrate-induced quenching of a fluorophore and its application in biosensor development

Ashutosh Sharma, Nigel S. M. Quantrill

Proceedings Volume 2131, Biomedical Fiber Optic Instrumentation; (1994) https://doi.org/10.1117/12.180764
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A novel process called substrate induced quenching (SIQ) and a mathematical derivation of an empirical relation describing it are presented. Based on this a model is proposed for a dehydrogenase enzymatic system that includes the effects due to dynamic/static quenching of the fluorophore by either the enzyme substrate or the product. The validity of the model is shown by comparison with experimental results for the SIQ based ethanol assay involving the alcohol dehydrogenase enzyme. Various applications of this novel technique are discussed.

Citation Download Citation

Ashutosh Sharma and Nigel S. M. Quantrill "Substrate-induced quenching of a fluorophore and its application in biosensor development", Proc. SPIE 2131, Biomedical Fiber Optic Instrumentation, (28 July 1994); https://doi.org/10.1117/12.180764

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