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26 May 1994 Video imaging of cardiac transmembrane activity
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High resolution movies of transmembrane electrical activity in thin (0.5 mm) slices of sheep epicardial muscle were recorded by optical imaging with voltage-sensitive dyes and a CCD video camera. Activity was monitored at approximately 65,000 picture elements per 2 cm2 tissue for several seconds at a 16 msec sampling rate. Simple image processing operations permitted visualization and analysis of the optical signal, while isochrome maps depicted complex patterns of propagation. Maps of action potential duration and regional intermittent conduction block showed that even these small preparations may exhibit considerable spatial heterogeneity. Self-sustaining reentrant activity in the form of spiral waves was consistently initiated and observed either drifting across the tissue or anchored to small heterogeneities. The current limitations of video optical mappings are a low signal-to- noise ratio and low temporal resolution. The advantages include high spatial resolution and direct correlation of electrical activity with anatomy. Video optical mapping permits the analysis of the electrophysiological properties of any region of the preparation during both regular stimulation and reentrant activation, providing a useful tool for studying cardiac arrhythmias.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
William T. Baxter, Jorge Davidenko, Candido Cabo, and Jose Jalife "Video imaging of cardiac transmembrane activity", Proc. SPIE 2132, Clinical Applications of Modern Imaging Technology II, (26 May 1994);

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