Studies on porphyrin photoproducts in solution, cells, and tumor tissue

Karsten Koenig; Herbert Schneckenburger; Angelika C. Rueck; Roland Koenig

doi:10.1117/12.179988

19 July 1994 Studies on porphyrin photoproducts in solution, cells, and tumor tissue

Karsten Koenig, Herbert Schneckenburger, Angelika C. Rueck, Roland Koenig

Proceedings Volume 2133, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy III; (1994) https://doi.org/10.1117/12.179988
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Light excitation of photosensitizing porphyrins leads to cytotoxic reactions. In addition, photobleaching and photoproduct formation occur indicating photosensitizer destruction. Photoproducts from hematoporphyrin (HP) fluoresce in aqueous solution at 642 nm, whereas photoproducts from protoporphyrin (PP) in hydrophobic environment emit around 670 nm and exhibit pronounced absorption at 665 nm. Photoproduct formation depends on singlet oxygen. The photoproducts exhibit faster fluorescence decay kinetics compared with nonirradiated porphyrins, as shown by time-grated spectroscopy and fluorescence decay measurements. Photoproduct fluorescence was observed during light exposure of cells and of tumor-bearing, nude mice, following administration of Hematoporphyrin Derivative (HpD), tetramethyl-HP, and PP. Photoconversion was also detected with naturally-occurring porphyrins (PP-producing bacteria) and ALA-simulated biosynthesis of PP in tumor tissue and in skin lesions of patients (psoriasis, mycosis fungoides). The efficiency of PDT with porphyrin photoproducts was found to be low in spite of the strong electronic transitions in the red spectral region.

Citation Download Citation

Karsten Koenig, Herbert Schneckenburger, Angelika C. Rueck, and Roland Koenig "Studies on porphyrin photoproducts in solution, cells, and tumor tissue", Proc. SPIE 2133, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy III, (19 July 1994); https://doi.org/10.1117/12.179988

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