19 July 1994 Studies on porphyrin photoproducts in solution, cells, and tumor tissue
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Abstract
Light excitation of photosensitizing porphyrins leads to cytotoxic reactions. In addition, photobleaching and photoproduct formation occur indicating photosensitizer destruction. Photoproducts from hematoporphyrin (HP) fluoresce in aqueous solution at 642 nm, whereas photoproducts from protoporphyrin (PP) in hydrophobic environment emit around 670 nm and exhibit pronounced absorption at 665 nm. Photoproduct formation depends on singlet oxygen. The photoproducts exhibit faster fluorescence decay kinetics compared with nonirradiated porphyrins, as shown by time-grated spectroscopy and fluorescence decay measurements. Photoproduct fluorescence was observed during light exposure of cells and of tumor-bearing, nude mice, following administration of Hematoporphyrin Derivative (HpD), tetramethyl-HP, and PP. Photoconversion was also detected with naturally-occurring porphyrins (PP-producing bacteria) and ALA-simulated biosynthesis of PP in tumor tissue and in skin lesions of patients (psoriasis, mycosis fungoides). The efficiency of PDT with porphyrin photoproducts was found to be low in spite of the strong electronic transitions in the red spectral region.
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Karsten Koenig, Karsten Koenig, Herbert Schneckenburger, Herbert Schneckenburger, Angelika C. Rueck, Angelika C. Rueck, Roland Koenig, Roland Koenig, } "Studies on porphyrin photoproducts in solution, cells, and tumor tissue", Proc. SPIE 2133, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy III, (19 July 1994); doi: 10.1117/12.179988; https://doi.org/10.1117/12.179988
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