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19 May 1994 In-vivo fluorescence imaging of normal and tumorous tissue using a pH-sensitive probe
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This study aimed to develop a spectroscopic imaging method to visualize superficial pH of tumor and normal tissues after injection of 5,6-carboxyfluorescein as pH marker. In vivo experiments were performed on CDF mice bearing a lymphoid leukemia P388 grafted tumor. Tissue pH were controlled by microelectrodes. Metabolic depression of tumor pH was induced by prior injection of glucose (6g/kg). Images show lower fluorescence intensity in tumor. Both fluorescence kinetic show an increase followed by a plateau phase. Calculated ratio were 1.72+/- 0.07 and 2.03+/- 0.04 in tumor and normal tissues respectively. These ratios based on a calibration curve correspond to 6.21+/- 0.12 and 6.99+/- 0.16. Controlled pH by microelectrodes show similar values (6.2+/- 0.3 and 7.0+/- 0.2 respectively). This imaging technique can be a new tool to study metabolic and functional event in tumor like the relation between pH and hypoxia.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Serge R. Mordon, Jean-Marie Devoisselle, and Vincent Maunoury M.D. "In-vivo fluorescence imaging of normal and tumorous tissue using a pH-sensitive probe", Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); doi: 10.1117/12.176011;

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