Photodynamic treatment (PDT) of endometrium primary cultures serving as an in-vitro-model for endometriosis

Wiebke Herter; Volker Viereck; J. Keckstein M.D.; Rudolf W. Steiner; Angelika C. Rueck

doi:10.1117/12.176005

19 May 1994 Photodynamic therapy (PDT) of endometrium primary cultures serving as an in-vitro model for endometriosis

Wiebke Herter, Volker Viereck, J. Keckstein M.D., Rudolf W. Steiner, Angelika C. Rueck

Proceedings Volume 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases; (1994) https://doi.org/10.1117/12.176005
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

As a new treatment model for endometriosis, photodynamic therapy (PDT) was applied to endometrium cultures. Endometriosis is a benign disease. Therefore primary cultures were used instead of cell lines. Endometrium is composed of epithelial and stromal cells which can also be found in primary culture. While stromal cells take a polygonal shape in culture, epithelial cells form cell colonies. PSIII (Photasan III), which is similar to hematorporphyrin derivate (HpD), meso-tetra (4-sulfonatophenyl) porphyrin (TPPS₄), which posses a high fluorescence quantum yield and may be useful in fluorescence diagnosis of subtle endometriotic spots, and methylene blue (MB), a vital dye with phototoxic properties, were used as photosensitizers. Different sensitizer concentrations and incubation times were applied. The highest phototoxicity was observed for PSIII; TPPS₄ and MB were less phototoxic. We compared our results with the sensitivity of cell lines described in the literature. The necessary irradiation to destroy stromal cells was relatively high but still in the same dimension as for cell lines. However they were even more sensitive than epithelial cells. This was true for all sensitizers used.

Citation Download Citation

Wiebke Herter, Volker Viereck, J. Keckstein M.D., Rudolf W. Steiner, and Angelika C. Rueck "Photodynamic therapy (PDT) of endometrium primary cultures serving as an in-vitro model for endometriosis", Proc. SPIE 2135, Advances in Laser and Light Spectroscopy to Diagnose Cancer and Other Diseases, (19 May 1994); https://doi.org/10.1117/12.176005

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