Diagnostics of physiological states of organism by means of fluorescent measurement of membrane potential of blood cells

Tatyana V. Vengrus; Galina Zatsepina; Svetlana V. Egudina; Nikolay N. Goryunov; Olga V. Tarnopolskaya

doi:10.1117/12.180784

21 July 1994 Diagnostics of physiological states of organism by means of fluorescent measurement of membrane potential of blood cells

Tatyana V. Vengrus, Galina Zatsepina, Svetlana V. Egudina, Nikolay N. Goryunov, Olga V. Tarnopolskaya

Author Affiliations +

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180784
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Average membrane potential (MP) of T-lymphocytes in different physiological states of mice were measured with the help of fluorescent probe anilinonaphtalin-8-sulphonate (ANS). We investigated thymocytes of healthy animals and animals in whose organisms inflammatory process (IP) or cancer (IC) was developed. It was shown that MP is different in different physiological states of mice and is 61 mV, 96 mV, and 112 mV in control, IP, and IC respectively. Kinetics of regulation of volume and MP of T-lymphocytes in different physiological states in hypotonic medium were also investigated. It was shown that the kinetics have different quasiperiodic characters in the case of healthy organism and in the case of IP. During development of IC T-lymphocytes lost their adaptability to hypotension. The disappearance of adaptability of T-lymphocytes beginning from the third day of development of IC represents an early test of cancer. The relationship between MP and surface potential of plasmatic membrane was also investigated.

Citation Download Citation

Tatyana V. Vengrus, Galina Zatsepina, Svetlana V. Egudina, Nikolay N. Goryunov, and Olga V. Tarnopolskaya "Diagnostics of physiological states of organism by means of fluorescent measurement of membrane potential of blood cells", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180784

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available