Large-area waveguide sensor for multiple analytes detection

Winston Ho; Peter W. Low; Daniel P. Robinson

doi:10.1117/12.180803

21 July 1994 Large-area waveguide sensor for multiple analytes detection

Winston Ho, Peter W. Low, Daniel P. Robinson

Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180803
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

A highly sensitive fluoroimmunoassay optical waveguide for the monitoring of biological agents was developed. The scope and versatility of this method was enhanced by combining the principle of fluoroimmunoassay with latex-based waveguide evanescent wave sensing technology. A novel waveguide probe was successfully demonstrated as an antibody-based biosensor. Based on a designed biological model, human immunoglobulin G (h-IgG) were sensitively (0.3 ng/ml, 2 X 10^-12 M) and rapidly (2 minutes assay time) identified and quantified using a diode laser (635 nm). The latex-based thin film has excellent optical quality and an established immunochemistry, making it stable and reliable for sensing applications. Because polymer-matrix waveguide is inexpensive and disposable, the probe cartridge is suitable for one time assay. Very fast and highly sensitive biosensors are potentially useful for many medical and clinical diagnostics, especially for intensive or emergency care patients.

Citation Download Citation

Winston Ho, Peter W. Low, and Daniel P. Robinson "Large-area waveguide sensor for multiple analytes detection", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); https://doi.org/10.1117/12.180803

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