21 July 1994 Novel optical method for measuring D-glucose 6-phosphate
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Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180780
Event: OE/LASE '94, 1994, Los Angeles, CA, United States
Abstract
A novel D-glucose 6-phosphate (G6P) assay is described that exploits the technique of substrate induced quenching. In a reaction between G6P and glucose 6-phosphate dehydrogenase (G6P-DH) the cofactor nicotinamide adenine dinucleotide (NAD+) is reduced to NADH. The latter being a quencher of thionine allows an indirect determination of the G6P concentration. A Stern-Volmer type analysis of the data for the investigated range of 0 - 1 mM G6P results in a quenching constant of 439 (+/- 37) M-1, with a detection limit of 9 (mu) M. A simple empirical model based on enzyme kinetics is presented for the overall G6P assay. Excellent correlation exists between the results predicted by the model and those obtained experimentally. Possibilities of employing the proposed G6P SIQ assay in multi-enzyme assays and in the construction of immunoassays is discussed.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ashutosh Sharma, Nigel S. M. Quantrill, "Novel optical method for measuring D-glucose 6-phosphate", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180780; https://doi.org/10.1117/12.180780
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