21 July 1994 Substrate-induced quenching-based measurement of glycerol
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Proceedings Volume 2136, Biochemical Diagnostic Instrumentation; (1994) https://doi.org/10.1117/12.180781
Event: OE/LASE '94, 1994, Los Angeles, CA, United States
Abstract
Glycerol concentrations are measured using a novel photo reaction between an excited fluorophore and NADH, the latter being generated due to the reduction of NAD+ in an enzymatic reaction between glycerol dehydrogenase (GlDH) and glycerol. The results are treated using a modified Stern-Volmer type relation and at equilibrium, a SIQ quenching constant of 9.78 (+/- 2.05) M-1 is obtained for the substrate induced quenching of thionine by glycerol. The reported method was investigated over the glycerol concentration range of 0 - 100 mM and offers a detection limit of 0.4 mM.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Ashutosh Sharma, Ashutosh Sharma, Nigel S. M. Quantrill, Nigel S. M. Quantrill, } "Substrate-induced quenching-based measurement of glycerol", Proc. SPIE 2136, Biochemical Diagnostic Instrumentation, (21 July 1994); doi: 10.1117/12.180781; https://doi.org/10.1117/12.180781
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