Interaction of atherogenic lipoproteins with cultured cells: a confocal laser scanning microscopy study

Gerald Hofer; Roland Gorges; Fritz Paltauf; Gerhard M. Kostner; Albin Hermetter

doi:10.1117/12.182733

17 August 1994 Interaction of atherogenic lipoproteins with cultured cells: a confocal laser scanning microscopy study

Gerald Hofer, Roland Gorges, Fritz Paltauf, Gerhard M. Kostner, Albin Hermetter

Proceedings Volume 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV; (1994) https://doi.org/10.1117/12.182733
Event: OE/LASE '94, 1994, Los Angeles, CA, United States

Abstract

Low density lipoprotein (LDL) and lipoprotein (a) [Lp(a)] were covalently labeled with the fluorescent dyes BODIPY succinimidyl ester (green) or Rhodamine iodoacetamide (red). The interaction of the fluorescent lipoproteins with HepG2 cells was visualized by means of a confocal laser scanning fluorescence microscope operating in the dual wavelength mode. If LDL or Lp(a) were incubated with the cells both lipoproteins bound to the cell surface at 4 degree(s)C or were internalized by the cells at 37 degree(s)C. In all cases larger amounts of LDL interacted with the cells compared with Lp(a). When mixtures of LDL and Lp(a), each labeled with a different dye, were incubated with cells again both lipoproteins bound to the cell surface (4 degree(s)C) or were internalized by the cells (37 degree(s)C). In addition, the major part of the lipoproteins colocalized either on the cell surface or inside the cells. thus, we conclude that interactions of Lp(a) with cells is mediated by LDL, probably via the LDL receptor, to a large extent.

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Gerald Hofer, Roland Gorges, Fritz Paltauf, Gerhard M. Kostner, and Albin Hermetter "Interaction of atherogenic lipoproteins with cultured cells: a confocal laser scanning microscopy study", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); https://doi.org/10.1117/12.182733

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KEYWORDS

Luminescence

Receptors

Confocal microscopy

Proteins

Confocal laser scanning microscopy

Rhodamine

Microscopy

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