17 August 1994 One- and two-pulse theory of light quenching
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Abstract
We have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high-repetition rate lasers, a phenomenon we call 'light quenching'. We now describe some of the possible effects of light quenching on the time- resolved intensity and anisotropy decays of fluorophores. We show that light quenching can decrease or increase the time-zero anisotropy, and that the initial anisotropies can increase to unity under selected conditions. Quenching with time-delayed light pulses is predicted to result in oscillations in the frequency-domain intensity and anisotropy decays. The increasing available and use of pulsed laser sources of light offers the opportunity for a new class of one- two- or multiple-pulse time- resolved experiments.
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Jozef Kusba, Valery L. Bogdanov, Ignacy Gryczynski, Joseph R. Lakowicz, "One- and two-pulse theory of light quenching", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182737; https://doi.org/10.1117/12.182737
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