17 August 1994 One- and two-pulse theory of light quenching
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We have recently demonstrated that fluorescence emission can be quenched by laser light pulses from modern high-repetition rate lasers, a phenomenon we call 'light quenching'. We now describe some of the possible effects of light quenching on the time- resolved intensity and anisotropy decays of fluorophores. We show that light quenching can decrease or increase the time-zero anisotropy, and that the initial anisotropies can increase to unity under selected conditions. Quenching with time-delayed light pulses is predicted to result in oscillations in the frequency-domain intensity and anisotropy decays. The increasing available and use of pulsed laser sources of light offers the opportunity for a new class of one- two- or multiple-pulse time- resolved experiments.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Jozef Kusba, Jozef Kusba, Valery L. Bogdanov, Valery L. Bogdanov, Ignacy Gryczynski, Ignacy Gryczynski, Joseph R. Lakowicz, Joseph R. Lakowicz, } "One- and two-pulse theory of light quenching", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182737; https://doi.org/10.1117/12.182737


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