17 August 1994 Photophysical properties of nucleic acids that contain A and T bases, and global analysis of steady-state fluorescence spectra in the presence of energy transfer
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Abstract
We have studied the steady-state fluorescence properties of the polynucleotides poly(dA)poly(dT) and poly(dT) as well as of the 20-mers (dA)20(dT)20 and (dT)20 at room temperature for excitation at 293 nm where T is selectively excited. The fluorescence spectrum of the double-stranded 20-mer is shifted to longer wavelengths relative to that of poly(dA)poly(dT), which may be the result of reduced stacking interactions. The fluorescence spectrum of the single-stranded 20-mer is much broader than that of poly(dT); this casts doubt on the validity of the assumption made in the literature that the crystallographic results from a dimer can be used to develop a model for poly(dT). The fluorescence anisotropies of the double- stranded systems are unexpectedly lower than those of the single- stranded ones, which suggests that cooperative interactions operate in the dynamics of the former. The analysis of the results of these systems was considerably simplified because of the fact that T can be selectively excited at the long-wavelength edge of the absorption spectrum. For the more general case for which such a spectral window does not exist, we have developed a methodology that employs a global nonlinear least-squares analysis of the dependence of the fluorescence intensity on the polarization angle and have estimated, for a simulated mixture of the C and G bases, their individual fluorescence spectra in the presence of energy transfer from C to G as well as from G to C.
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Solon Georghiou, Gregory Phillips, L. S. Taylor-Gerke, Alexander Philippetis, T. D. Bradrick, "Photophysical properties of nucleic acids that contain A and T bases, and global analysis of steady-state fluorescence spectra in the presence of energy transfer", Proc. SPIE 2137, Time-Resolved Laser Spectroscopy in Biochemistry IV, (17 August 1994); doi: 10.1117/12.182778; https://doi.org/10.1117/12.182778
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