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11 May 1994 Quantitative analysis of volume images: electron microscopic tomography of HIV
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Three-dimensional objects should be represented by 3D images. So far, most of the evaluation of images of 3D objects have been done visually, either by looking at slices through the volumes or by looking at 3D graphic representations of the data. In many applications a more quantitative evaluation would be valuable. Our application is the analysis of volume images of the causative agent of the acquired immune deficiency syndrome (AIDS), namely human immunodeficiency virus (HIV), produced by electron microscopic tomography (EMT). A structural analysis of the virus is of importance. The representation of some of the interesting structural features will depend on the orientation and the position of the object relative to the digitization grid. We describe a method of defining orientation and position of objects based on the moment of inertia of the objects in the volume image. In addition to a direct quantification of the 3D object a quantitative description of the convex deficiency may provide valuable information about the geometrical properties. The convex deficiency is the volume object subtracted from its convex hull. We describe an algorithm for creating an enclosing polyhedron approximating the convex hull of an arbitrarily shaped object.
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Ingela Nystroem, Ewert W. Bengtsson, Bo G. Nordin, and Gunilla Borgefors "Quantitative analysis of volume images: electron microscopic tomography of HIV", Proc. SPIE 2167, Medical Imaging 1994: Image Processing, (11 May 1994);

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