Acquisition of low-light-level biological images using an image intensified CCD camera for real-time quantitative analysis

Li Xu; Calum E. MacAulay

doi:10.1117/12.175150

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5 May 1994 Acquisition of low-light-level biological images using an image intensified CCD camera for real-time quantitative analysis

Li Xu, Calum E. MacAulay

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Proceedings Volume 2173, Image Acquisition and Scientific Imaging Systems; (1994) https://doi.org/10.1117/12.175150
Event: IS&T/SPIE 1994 International Symposium on Electronic Imaging: Science and Technology, 1994, San Jose, CA, United States

Abstract

The characteristics of a computer controlled intensified CCD camera system are quantitatively studied. The light response of the computer controlled camera system is found to be non- linearly dependent upon the gains of the intensifier, the CCD amplifier, and the imaging board. Controlling the intensifier gain is considered the most efficient method of controlling the system's sensitivity and is the chosen working mode. A 3D analytical model of the camera's light response is generated. An inverse transformation was designed. By employing a look-up table (LUT) to map the inverse transformation using hardware in the imaging board, the non-linearity of the camera's light response at any given operating point can be corrected in real time.

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Li Xu and Calum E. MacAulay "Acquisition of low-light-level biological images using an image intensified CCD camera for real-time quantitative analysis", Proc. SPIE 2173, Image Acquisition and Scientific Imaging Systems, (5 May 1994); https://doi.org/10.1117/12.175150

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