4 April 1994 Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy
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Abstract
Axial resolution in fluorescence microscopy can be improved significantly by using standing wave illumination to selectively excite planes within the depth of field of the microscope. When the specimen is thinner than 0.18 micrometers , an estimate of its 3D structure may be determined from three images within the same focal plane without re-focusing. Thicker objects require a combination of multi-focal-plane data and/or a priori knowledge.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Brent Bailey, Vijay Krishnamurthi, Daniel L. Farkas, D. Lansing Taylor, Frederick Lanni, "Three-dimensional imaging of biological specimens with standing wave fluorescence microscopy", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172101; https://doi.org/10.1117/12.172101
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KEYWORDS
Microscopy

Luminescence

3D image processing

Microscopes

Refraction

Image resolution

Yeast

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