4 April 1994 Two-photon excitation in time-resolved fluorescence microscopy
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Resonant two photon excitation of UV absorbing dyes with red light is a new concept in 3D microscopy. This technique provides functionality which is virtually equivalent to a confocal microscope. Two photon excitation allows excitation of UV dyes without special UV corrected optics. Resonant two photon excitation in microscopy, as known until now, utilizes expensive, high powered pulsed femto- or picosecond lasers. In this paper a cost effective alternative to resonant two photon is presented.
© (1994) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Pekka E. Hanninen, Pekka E. Hanninen, Stefan W. Hell, Stefan W. Hell, Ari Kuusisto, Ari Kuusisto, Lotta Lehtela, Lotta Lehtela, Juha Salo, Juha Salo, Erkki J. Soini, Erkki J. Soini, } "Two-photon excitation in time-resolved fluorescence microscopy", Proc. SPIE 2184, Three-Dimensional Microscopy: Image Acquisition and Processing, (4 April 1994); doi: 10.1117/12.172107; https://doi.org/10.1117/12.172107

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