Investigations of hematoporphyrine emission properties

Miroslaw Kwasny; Zygmunt Mierczyk; Alfreda Graczyk

doi:10.1117/12.203332

1 March 1995 Investigations of hematoporphyrine emission properties

Miroslaw Kwasny, Zygmunt Mierczyk, Alfreda Graczyk

Proceedings Volume 2203, Laser Technology IV: Applications in Medicine; (1995) https://doi.org/10.1117/12.203332
Event: Laser Technology: Fourth Symposium, 1993, Szczecin, Poland

Abstract

In this paper the dimerization constant of pure hematoporphyrine was established using absorption method and then fluorescence quantum yield ((phi) ) was calculated as a function of monomeric fraction ((alpha) ). It was our objective to formulate an equation describing the relationships between (phi) , (alpha) and ratio of monomer to dimer molar absorption coefficients ((epsilon) _M/(epsilon) _D), at a given wavelength. (phi) was also studied as a function of pH value and polarity. A 3-D stack fluorescence plot was also constructed and the possibilities of practical application of the fluorescence effect to the visualization of areas with absorbed are presented.

Citation Download Citation

Miroslaw Kwasny, Zygmunt Mierczyk, and Alfreda Graczyk "Investigations of hematoporphyrine emission properties", Proc. SPIE 2203, Laser Technology IV: Applications in Medicine, (1 March 1995); https://doi.org/10.1117/12.203332

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