Three-dimensional reconstruction of ocean chlorophyll distributions from underwater serial sectioned fluorescence images

Andrew W. Palowitch; Jules S. Jaffe

doi:10.1117/12.190103

26 October 1994 Three-dimensional reconstruction of ocean chlorophyll distributions from underwater serial sectioned fluorescence images

Andrew W. Palowitch, Jules S. Jaffe

Author Affiliations +

Proceedings Volume 2258, Ocean Optics XII; (1994) https://doi.org/10.1117/12.190103
Event: Ocean Optics XII, 1994, Bergen, Norway

Abstract

An underwater optical serial sectioning system has been developed to measure in-situ three- dimensional distributions of biological particles. The technique involves scanning a thin plane of laser light sequentially through a range of distances parallel to the imaging plane of a digital CCD camera. Images of induced fluorescence in the sequentially illuminated planes are recorded. A computer based inverse program is then used to reconstruct three-dimensional chlorophyll (alpha) distributions from the plane images. The inverse method compensates for attenuation of the fluorescence excitation and emission throughout the volume and converts camera image plane intensity values to mg Chl (alpha) /m³. Graphic display of the composite data set with spatial resolution of 1 cm³ in a 20 X 20 X 20 cm volume is presented in a three-dimensional rendered volume. Chl (alpha) concentration discrimination of 0.1 mg Chl (alpha) /m³ over the range of 0.1 to 2.0 mg Chl (alpha) /m³ has been demonstrated in the laboratory with the optical serial sectioning system.

Citation Download Citation

Andrew W. Palowitch and Jules S. Jaffe "Three-dimensional reconstruction of ocean chlorophyll distributions from underwater serial sectioned fluorescence images", Proc. SPIE 2258, Ocean Optics XII, (26 October 1994); https://doi.org/10.1117/12.190103

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available