Translator Disclaimer
18 January 1995 Laser-induced release of liposome-encapsulated dye to monitor tissue temperature: study of different liposome compositions
Author Affiliations +
Proceedings Volume 2323, Laser Interaction with Hard and Soft Tissue II; (1995) https://doi.org/10.1117/12.199211
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France
Abstract
This study aimed to evaluate the interest of several liposome compositions (DPPC, DSPC, DPPA) to control specific ranges of temperature and to assess the possible use of temperature sensitive liposomes in an established model such as the liver as a new approach to monitor tissue temperature under laser irradiation. Temperature sensitive liposomes (DPPC or DSPC or DPPA) loaded with carboxy-fluorescein were injected to Wistar rats. The liver was exposed and irradiated with a 100 W Nd:YAG laser (single pulse mode, pulses ranging from 100 to 260 ms, spot diameter: 4 mm) to avoid direct absorption by the dye entrapped in the liposomes. The temperature was measured with an infrared camera during laser irradiation. The animals were then sacrificed and the liver was surgically removed. Immediately after, the fluorescence was measured -- ex vivo -- with a fluorescent imaging system. We were not able to prepare stable high transition temperature liposomes (DPPE). Concerning DPPC, the mechanism of dye release at the basal temperature led to a complete leakage of the dye in less than 5 minutes. Only background fluorescence was observed but no specific response due to laser irradiation. Nevertheless the results obtained using DSPC liposomes meet to a large extent our requirements since a useful monitoring of temperature is feasible from 42 degree(s)C to 62 degree(s)C. In fact the critical temperature of most tissues varies from 53 degree(s)C to 58 degree(s)C.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Thomas Desmettre, Serge R. Mordon, Jean-Marie Devoisselle, and Sylvie Soulie-Begu "Laser-induced release of liposome-encapsulated dye to monitor tissue temperature: study of different liposome compositions", Proc. SPIE 2323, Laser Interaction with Hard and Soft Tissue II, (18 January 1995); doi: 10.1117/12.199211; https://doi.org/10.1117/12.199211
PROCEEDINGS
8 PAGES


SHARE
Advertisement
Advertisement
Back to Top