Evaluation of changes in the NADH level between carcinogenic and normal tissue samples by use of fluorescence spectroscopy

Volkmar Betz; Herbert Schneckenburger; H. P. Alleroeder; Gerhard W. Sybrecht; Joerg-Uwe Meyer

doi:10.1117/12.198733

4 January 1995 Evaluation of changes in the NADH level between carcinogenic and normal tissue samples by use of fluorescence spectroscopy

Volkmar Betz, Herbert Schneckenburger, H. P. Alleroeder, Gerhard W. Sybrecht, Joerg-Uwe Meyer

Author Affiliations +

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198733
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

The metabolism of cancer cells frequently shows higher glycolytic activity than normal tissue. This glycolytic activity can result in changes of the NAD⁺/NADH equilibrium and variations of NAD^{+$ and NADH levels. The objective of this study has been to monitor changes in NADH fluorescence spectra of cancerous and normal bronchial tissue. Spectra were taken from in- vitro samples of normal and carcinogenic bronchial tissue in a microspektrofluorometric setup and in a spectrofluorometer, the latter giving the Emission-Excitation-Matrix (EEM} of the tissue fluorescence. Two numerical approaches were taken to determine changes in the NADH spectra. (1) Graphical comparison of normalized emission spectra shows a blue shift of the maximum for carcinogenic tissue. There is a good correspondence between the form of the spectrum and the spectrum of LDH-bound NADH. (2) Numerical analysis of the EEM from freeze slices showed an increased signal in the case of normal tissue and a decreased one in the case of cancerous samples in the spectral range that is typical for NADH.

Citation Download Citation

Volkmar Betz, Herbert Schneckenburger, H. P. Alleroeder, Gerhard W. Sybrecht, and Joerg-Uwe Meyer "Evaluation of changes in the NADH level between carcinogenic and normal tissue samples by use of fluorescence spectroscopy", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198733

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