Fluorescence spectroscopy in combination with reflectance measurements in human skin examination: what for and how

Sergei R. Utz M.D.; Yurii P. Sinichkin; Igor Victorovich Meglinski; Helena A. Pilipenko

doi:10.1117/12.198712

4 January 1995 Fluorescence spectroscopy in combination with reflectance measurements in human skin examination: what for and how

Sergei R. Utz M.D., Yurii P. Sinichkin, Igor Victorovich Meglinski, Helena A. Pilipenko

Author Affiliations +

Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198712
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Reflectance and fluorescence spectroscopy are successfully used for skin disease diagnostics. Human skin optical parameters are defined by its turbid, scattering properties with nonuniform absorption and fluorescence chromophores distribution, its multilayered structure, and variability under different physiological and pathological conditions. Theoretical modeling of light propagation in skin could improve the understanding of these conditions and may be useful in the interpretation of in vivo reflectance and autofluorescence spectra. In the present work the temporal behavior of in vivo reflectance and autofluorescence (excitation wavelength 337 nm) spectra of human skin under ultraviolet irradiation and external mechanical pressure were investigated. Experimental results and Monte Carlo modeling of light distribution in skin were compared and demonstrated good agreements. Combination of diffuse reflectance and autofluorescence measurements is a very promising technique for precise erythema and pigmentation of the human skin evaluation.

Citation Download Citation

Sergei R. Utz M.D., Yurii P. Sinichkin, Igor Victorovich Meglinski, and Helena A. Pilipenko "Fluorescence spectroscopy in combination with reflectance measurements in human skin examination: what for and how", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198712

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available