Translator Disclaimer
4 January 1995 Properties of DNA-polyintercalating drugs studied by fluorescence lifetime techniques
Author Affiliations +
Proceedings Volume 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging; (1995) https://doi.org/10.1117/12.198728
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France
Abstract
Dimers of the fluorescent dye Oxazole Yellow (YOYO and POPO) are used for high sensitive DNA detection in gel electrophoresis. Upon binding to DNA they show a 3000 to 5000 fold enhancement of fluorescence. The binding constant of those dimers to dsDNA is between 108 M-1 and 109 M-1. This is due to the dye's ability to bisintercalate between adjacent DNA basepairs. We investigated the occurring forms of intercalation of YOYO to dsDNA in solutions of different ionic strength by fluorescence lifetime methods.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Stefan Winter and Liviu M. Popa "Properties of DNA-polyintercalating drugs studied by fluorescence lifetime techniques", Proc. SPIE 2324, Optical Biopsy and Fluorescence Spectroscopy and Imaging, (4 January 1995); https://doi.org/10.1117/12.198728
PROCEEDINGS
7 PAGES


SHARE
Advertisement
Advertisement
Back to Top