Lethal photosensitization of Helicobacter species

Charles E. Millson; Michael Wilson; Alexander J. MacRobert; Wendy Thurrell; Peter Mlkvy; Claire Davies; Stephen G. Bown

doi:10.1117/12.199142

12 January 1995 Lethal photosensitization of Helicobacter species

Charles E. Millson, Michael Wilson, Alexander J. MacRobert, Wendy Thurrell, Peter Mlkvy, Claire Davies, Stephen G. Bown

Proceedings Volume 2325, Photodynamic Therapy of Cancer II; (1995) https://doi.org/10.1117/12.199142
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France

Abstract

Helicobacter pylori (H. pylori) is associated with a large number of gastroduodenal disorders. Clearance of the bacteria has been shown to benefit patients with duodenal ulcers, gastric ulcers, and certain rare types of gastric tumors. Broad-spectrum antibiotics are the mainstay of current treatment strategies but side-effects, poor compliance, and drug resistance limit their usefulness. We sensitized H. pylori with toluidine blue, haematoporphyrin derivative, aluminum disulphonated phthalocyanine, methylene blue or protoporphyrin IX prior to exposure to low-power laser light from either a gallium aluminum arsenide laser or a helium neon gas laser. All 5 sensitizers caused reductions of greater than 1000-fold in the number of viable bacteria. Light alone had no effect and only HpD caused a significant decrease in bacterial numbers without laser light. Next, we sensitized H. mustelae on explanted ferret gastric mucosa (ex vivo) with the same sensitizers and exposed them to light from a copper vapor pumped dye laser tuned appropriately. MB caused significant reductions in bacterial counts. Successful lethal photosensitization of Helicobacter pylori both in vitro and ex vivo raises the possibility of a local method for eradicating the bacteria, especially as the bacteria are only found in those parts of the upper gastrointestinal tract that are accessible to the endoscope.

Citation Download Citation

Charles E. Millson, Michael Wilson, Alexander J. MacRobert, Wendy Thurrell, Peter Mlkvy, Claire Davies, and Stephen G. Bown "Lethal photosensitization of Helicobacter species", Proc. SPIE 2325, Photodynamic Therapy of Cancer II, (12 January 1995); https://doi.org/10.1117/12.199142

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