1 February 1995 Optimizing DNA staining by Hoechst 33342 for assessment of chromatin organization in living cells
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Proceedings Volume 2329, Optical and Imaging Techniques in Biomedicine; (1995) https://doi.org/10.1117/12.200902
Event: International Symposium on Biomedical Optics Europe '94, 1994, Lille, France
Abstract
In recent years, there has been an increasing interest for applications of fluorescence measurements to studies on many physiological mechanisms in living cells. However, few studies have taken advantage of DNA quantification by fluorometry for dynamic assessment of chromatin organization. This type of approach involves both optimal conditions for DNA staining and the use of several investigation methods such as flow cytometry, image cytometry, laser scanning confocal microscopy and spectral imaging. In this context, this report describes a stoichiometric method for nuclear DNA specific staining, using bisbenzimidazole Hoechst 33342 associated with verapamil, a calcium membrane channel blocker. This method makes it possible to follow variations of nuclear DNA content in cells that are maintained alive.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Sylvain Paillasson, Sylvain Paillasson, Michel Robert-Nicoud, Michel Robert-Nicoud, Xavier Ronot, Xavier Ronot, } "Optimizing DNA staining by Hoechst 33342 for assessment of chromatin organization in living cells", Proc. SPIE 2329, Optical and Imaging Techniques in Biomedicine, (1 February 1995); doi: 10.1117/12.200902; https://doi.org/10.1117/12.200902
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