Aiming at the actual required of a body-in-white visual inspection system, and according to features of two structured light sensors (stripe light and multi-stripe light sensors) used in the system, this paper proposes a global calibration method using the direct linear transformation based on describing mathematical models of two kinds of sensors in brief and the multi-visual inspection system calibration in detail. The accomplishment of the calibrating process is presented on available condition. We design a corresponding calibrating device, and solve the sighting problem between target and structured light. A series of experiments have been finished. The results of them prove that the global calibration method is feasible and practical.