Particle size and scattering cross-section effects in laser Doppler electrophoresis of biological cells

Vadim L. Kononenko

doi:10.1117/12.197463

2 January 1995 Particle size and scattering cross-section effects in laser Doppler electrophoresis of biological cells

Vadim L. Kononenko

Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197463
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

The influence of the correlation between the size and electrophoretic mobility of particles on the shape of laser Doppler electrophoresis spectrum is considered. The theory of spectrum shape in the presence of such correlation is developed. It is shown that the oscillatory dependence of a light scattering cross-section of large particles on their size may distort substantially the spectrum shape corresponding solely to the electrophoretic mobility distribution in a particles system. Even the appearance of `false' line structure in the spectrum is possible for polydisperse particles systems with smooth distribution curves but with a high degree of size-electrophoretic mobility correlation. The theory explains naturally the oscillation structure observed in Doppler electrophoresis spectra of certain large cells.

Citation Download Citation

Vadim L. Kononenko "Particle size and scattering cross-section effects in laser Doppler electrophoresis of biological cells", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197463

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