Polarization properties of v10, v11, and v19 modes of (de)oxyheme of hemoglobin as obtained from three-color CSRS

Artemy Voroshilov; Gerald W. Lucassen; Cees Otto; Jan Greve

doi:10.1117/12.197440

2 January 1995 Polarization properties of v₁₀, v₁₁, and v19 modes of (de)oxyheme of hemoglobin as obtained from three-color CSRS

Artemy Voroshilov, Gerald W. Lucassen, Cees Otto, Jan Greve

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Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197440
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus

Abstract

We report polarization sensitive coherent Stokes Raman scattering (CSRS) measurements of oxy- and deoxyhemoglobin in aqueous solutions that were carried out under electronic resonance with the Q absorption bands. All independent susceptibility (Chi) ⁽³) components as well as anisotropic and anti-symmetric scattering contributions were resolved within frequency nondegenerate CSRS scheme. Eight bands of oxy- and five of deoxyhemoglobin were observed in the range 1500 - 1680 cm^-1. Each set of dispersion profiles was simultaneously fitted with a set of parameters including band positions, widths, amplitudes, phases and, importantly, all the CSRS depolarization ratios. On this basis the major bands were assigned to non-totally symmetric (nu) ₁₀, (nu) ₁₁, and (nu) ₁₉ modes of the porphyrin macrocycle. These displayed a clear correlation of vibrational phase with the mode symmetry. Of eight bands resolved in oxyHb spectra three were attributed to features of intermediate deoxyHb, caused by a partial photolysis of oxyhemes. On a nanosecond time scale they were found essentially similar to those found for stable deoxyHb. A detectable isotropy was observed for all non-totally symmetric modes of both oxy- and deoxyhemes. The (nu) ₁₀ and (nu) ₁₁ modes were found to exhibit anti-symmetry as well. The decrease in depolarization ratio (rho) ₁₂₁₂^R of anomalously polarized (nu) ₁₉ mode from 7.7 (oxyheme) to 4.3 (deoxyheme) was detected. The latter evidenced heme deformation related with a further doming that occurs upon release of oxygen.

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Artemy Voroshilov, Gerald W. Lucassen, Cees Otto, and Jan Greve "Polarization properties of v₁₀, v₁₁, and v19 modes of (de)oxyheme of hemoglobin as obtained from three-color CSRS", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197440

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KEYWORDS

Raman scattering

Raman spectroscopy

Polarization

Scattering

Absorption

Chromium

Anisotropy

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