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2 January 1995 Polarization properties of v10, v11, and v19 modes of (de)oxyheme of hemoglobin as obtained from three-color CSRS
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Proceedings Volume 2370, 5th International Conference on Laser Applications in Life Sciences; (1995) https://doi.org/10.1117/12.197440
Event: Laser Applications in Life Sciences: 5th International Conference, 1994, Minsk, Belarus
Abstract
We report polarization sensitive coherent Stokes Raman scattering (CSRS) measurements of oxy- and deoxyhemoglobin in aqueous solutions that were carried out under electronic resonance with the Q absorption bands. All independent susceptibility (Chi) (3) components as well as anisotropic and anti-symmetric scattering contributions were resolved within frequency nondegenerate CSRS scheme. Eight bands of oxy- and five of deoxyhemoglobin were observed in the range 1500 - 1680 cm-1. Each set of dispersion profiles was simultaneously fitted with a set of parameters including band positions, widths, amplitudes, phases and, importantly, all the CSRS depolarization ratios. On this basis the major bands were assigned to non-totally symmetric (nu) 10, (nu) 11, and (nu) 19 modes of the porphyrin macrocycle. These displayed a clear correlation of vibrational phase with the mode symmetry. Of eight bands resolved in oxyHb spectra three were attributed to features of intermediate deoxyHb, caused by a partial photolysis of oxyhemes. On a nanosecond time scale they were found essentially similar to those found for stable deoxyHb. A detectable isotropy was observed for all non-totally symmetric modes of both oxy- and deoxyhemes. The (nu) 10 and (nu) 11 modes were found to exhibit anti-symmetry as well. The decrease in depolarization ratio (rho) 1212R of anomalously polarized (nu) 19 mode from 7.7 (oxyheme) to 4.3 (deoxyheme) was detected. The latter evidenced heme deformation related with a further doming that occurs upon release of oxygen.
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Artemy Voroshilov, Gerald W. Lucassen, Cees Otto, and Jan Greve "Polarization properties of v10, v11, and v19 modes of (de)oxyheme of hemoglobin as obtained from three-color CSRS", Proc. SPIE 2370, 5th International Conference on Laser Applications in Life Sciences, (2 January 1995); https://doi.org/10.1117/12.197440
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