Transcutaneous photodynamic therapy delays the onset of paralysis in a murine multiple sclerosis model

David W. C. Hunt; Simon Leong; Julia G. Levy; Agnes H. Chan

doi:10.1117/12.203392

1 March 1995 Transcutaneous photodynamic therapy delays the onset of paralysis in a murine multiple sclerosis model

David W. C. Hunt, Simon Leong, Julia G. Levy, Agnes H. Chan

Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203392
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States

Abstract

Photodynamic therapy (PDT) using benzoporphyrin derivative (BPD, Verteporfin) and whole body irradiation, can affect the course of adoptively transferred experimental allergic (autoimmune) encephalomyelitis (EAE) in PL mice. Murine EAE is a T cell-mediated autoimmune disease which serves as a model for human multiple sclerosis. Using a novel disease induction protocol, we found that mice characteristically developed EAE within 3 weeks of receipt of myelin basic protein (MBP)-sensitized, in vitro-cultured spleen or lymph node cells. However, if animals were treated with PDT (1 mg BPD/kg bodyweight and exposed to whole body 15 Joules cm² of LED light) 24 hours after receiving these cells, disease onset time was significantly delayed. PDT-treated mice developed disease symptoms 45 +/- 3 days following cell administration whereas untreated controls were affected within 23 +/- 2 days. In contrast, application of PDT 48 or 120 hours following injection of the pathogenic cells had no significant effect upon the development of EAE. Experiments are in progress to account for the protective effect of PDT in this animal model. These studies should provide evidence on the feasibility of PDT as a treatment for human autoimmune disease.

Citation Download Citation

David W. C. Hunt, Simon Leong, Julia G. Levy, and Agnes H. Chan "Transcutaneous photodynamic therapy delays the onset of paralysis in a murine multiple sclerosis model", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203392

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