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1 March 1995 Wavelength-dependent photodynamic inactivation of HaCaT human keratinocytes after preincubation with 5-aminolevulinic acid
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Proceedings Volume 2371, 5th International Photodynamic Association Biennial Meeting; (1995) https://doi.org/10.1117/12.203421
Event: Fifth International Photodynamic Association Biennial Meeting, 1994, Amelia Island, FL, United States
Abstract
To determine the optimum wavelength for irradiation with coherent light after 5-ALA-induced protoporphyrin IX-sensitization, HaCaT cells were allowed to take up 5-ALA (30 (mu) g/ml) for 24 h. A tunable dye laser was used for PDT (40 mW/cm2, 30 J/cm2, wavelengths between 622 - 649 nm in steps of 3 nm). Afterwards, an MTT assay was performed to check cell viability (CV). Four groups (n equals 8) were formed: A equals controls, B equals irradiation only, C equals 5-ALA only, D equals 5-ALA and irradiation. CV of groups B, C, D were compared to those of group A (100%). Group B showed no significant difference (92% range at different wavelengths between 72% and 100%). Group D showed a significant decrease of CV (61%, range at different wavelengths between 35% and 86%). Lowest values for CV were detected at 634 and 637 nm. We believe therefore, that PDT with 5-ALA as sensitizer shows better cytotoxicity when wavelengths at 634 - 637 nm are used.
© (1995) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Rolf-Markus Szeimies, Wolfgang Baeumler, Sigrid Karrer, and Michael Landthaler "Wavelength-dependent photodynamic inactivation of HaCaT human keratinocytes after preincubation with 5-aminolevulinic acid", Proc. SPIE 2371, 5th International Photodynamic Association Biennial Meeting, (1 March 1995); https://doi.org/10.1117/12.203421
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